Biomaterials (2022) Chemically-induced osteogenic cells for bone tissue engineering and disease modeling
Yoon JY, Mandakhbayar N, Hyun J, ..., Lee JH*, Leong KW, Kim HW*
Bioactive Mater (2022) Dendritic cell-mimicking scaffolds for ex vivo T cell expansion
Kim HS, Ho TC, ..., Kim HW, Leong KW*
Adv Health Mater (2022) Mimicking Bone Extracellular Matrix: From BMP-2-Derived Sequences...
Oliver-Cervelló L, Martin-Gómez H, ...,Kim HW, Ginebra MP, Lee JH, Mas-Moruno C*
Biomaterials (2022) Diabetic bone regeneration with nanoceria-tailored scaffolds by recapitulating cellular microenvironment...
Singh RK, Yoon DS, Mandakhbayar N,... Lee JH*, Kim HW*
Bioactive Mater (2022) Materials and extracellular matrix rigidity highlighted in...
Park JH, Jo S, Lee JH, Lee HH, Knowles JC, Kim HW*
Bioeng. Trans. Med. (2022) Hyperelastic, shape‐memorable, and ultra‐cell‐adhesive...
Hong SM, Yoon JY, Cha JR, ..., Lee JH*, Kim HW*
Cell Death Diff (2022) TLR4 downregulation by the RNA-binding protein PUM1...
Yoon DS, ..., Lee JH, Kim HW*, Lee JW*
Trend Mol Med (2022) Leveraging cellular mechano-responsiveness for cancer therapy
Hyun J, Kim HW*
Bioactive Mater (2022) Multifunctional GelMA platforms with nanomaterials for advanced tissue therapeutics
Kurian AG, Singh RK*, Patel KD, Lee JH, Kim HW*
Biomaterials (2021) Dual actions of osteoclastic-inhibition and osteogenic-stimulation through strontium-releasing...
Lee NH, Kang MS, ... Lee JH*, Kim HW*
Signal Trans Target Therapy (2021) Spatiotemporal control of CRISPR/Cas9 gene editing
Zhou C, Zhang J, Lee JH, ..., Kim HW*, Tao Y*, Li M*
Biomaterials (2021) Electricity auto-generating skin patch promotes wound healing process by activation of mechanosensitive ion...
Kim TH, Jeon WY, Ji Y, ... Lee JH*, Kim HW*
Science Adv. (2021) Scaffold-mediated CRISPR-Cas9 delivery system for acute myeloid leukemia therapy
Ho TC, Kim HS, ... Becker MW, Leong KW*
Biomaterials (2021) Therapeutic tissue regenerative nanohybrids self-assembled...
Kim HS, Lee JH, ... Shin US*, Kim HW*
J. Hepatology (2021) Dysregulation of the ESRP2-NF2-YAP/TAZ axis promotes hepatobiliary...
Hyun J, Al Abo M, ... Diehl AM*
Chem. Eng. J. (2021) Optimally dosed nanoceria attenuates osteoarthritic degeneration ...
Dashnyam K, Lee JH*, Singh RK,..., Kim HW*
Biomaterials (2021) Antibacterial, proangiogenic, and osteopromotive nanoglass...
Seo JJ, MAndakhbayar N, .., Lee JH, Kim HW*
Prog. Mater. Sci. (2020) Materials roles for promoting angiogenesis in tissue regeneration
Lee JJ, Parthiban P, Jin GZ, Knowles JC, Kim HW*
Biomaterials (2020) Protein-reactive nanofibrils decorated with cartilage-derived decellularized extracellular matrix for osteochondral defects
Kim HS, Mandakhbaya N, Kim HW, Leong K, Yoo HS*
Adv. Funct. Mater. (2020) Molecularly Imprinted Polymers and Electrospinning: Manufacturing Convergence ...
Patel K, Kim HW, Knowles JC, Poma A*
- (2022.09. People) Dr. Rajendra Kumar Singh, Dr. Dong Suk Yoon and Dr. Nandin Mandakbayar at ITREN developed a ROS-responsive nanoceria decorated scaffold based on 3D printing for hastening regeneration of critical sized bone defects in diabetic animals
- Dr. Rajendra Kumar Singh, Dr. Dong Suk Yoon and Dr. Nandin Mandakbayar at ITREN developed ROS-responsive nanoceria decorated scaffold based on 3D printing for hastening regeneration of critical sized bone defects in diabetic animals. The study recently got published in journal Biomaterials (IF:15.304) under the title “Diabetic bone regeneration with nanoceria-tailored scaffolds by recapitulating cellular microenvironment: Activating integrin/TGF-β co-signaling of MSCs while relieving oxidative stress. (Read the article online: https://doi.org/10.1016/j.biomaterials.2022.121732).
Regenerating defective bones in diabetic patients is of boundless clinical importance. The elevated blood glucose levels and oxidative stress in defected bones hinders the neo bone regeneration process and hence developing a therapeutic biomaterial that modulates oxidative stress while supporting osteogenesis is of great interest. This motivated Dr. Rajendra Kumar Singh, Dr. Dong Suk Yoon and Dr. Nandin Mandakbayar to challenge the problem by fabricating nanoceria decorated 3D-printed PCL scaffolds as a biomaterial for bone therapeutics. The entire study was conducted under the guidance of Prof. Hae-Won Kim and Prof. Jung-Hwan Lee here at ITREN. The excellent ROS-responsiveness and the nano-topological cues provided by scaffolds directed MSCS to express higher levels of focal adhesion proteins, curvature-sensing membrane proteins and significantly higher levels of osteogenic differentiation through integrin-mediated TGF-β co-signaling activation pathway. Such regulatory effects in MSC were further proven invivo by implantation in critical-sized bone defects of diabetic animals. Together all, the studies revealed that the currently exploited nCe-scaffolds can be a promising drug- and cell-free therapeutic means to treat defective tissues like bone in diabetic conditions.
- (2022.09. People) Dr. Suk-Min Hong, Dr. Ji-Young Yoon, and Dr. Jae-Ryung Cha at ITREN developed a novel polycaprolactone-based polyurethane (PCL-PU) copolymers with shape-memory, hyperelasticity, and ultra-cell-adhesion properties intended for various tissue regeneration applications
- Dr. Suk-Min Hong, Dr. Ji-Young Yoon, and Dr. Jae-Ryung Cha at ITREN developed polycaprolactone-based polyurethane (PCL-PU) copolymers with excellent shape-memory, hyper-elasticity, and ultra-cell-adhesion properties intended for various tissue regeneration applications. The study recently got published in journal Bioengineering &Translational medicine (IF: 10.684) under the title “Hyperelastic, shape-memorable, and ultra-cell-adhesive degradable polycaprolactone-polyurethane copolymer for tissue regeneration” (Read the article online: https://doi.org/10.1002/btm2.10332).
The PCL-PU biomaterial developed have shown prominent mechanical properties (~ 50 MPa tensile strength and ~ 1150% elongation with hyper-elasticity under cyclic load). The shape-memory features were also proved in film, thread, and 3D scaffold forms. With extensive invitro experiments, authors revealed the ultra-cell-adhesive properties and tissue regenerative potential by performing differentiation towards myogenic and osteogenic lineages. Furthermore, tissue compatibility, immune responses, and regenerative potential was investigated in-vivo. This study suggests the multifunctional roles of PCL-PU as a therapeutic biomaterial exclusively for minimally invasive surgeries that demands minor skin openings to target large defects along with promoting excellent tissue regeneration.
- (2022.09. People) Dr. Ji-Young-Yoon at ITREN successfully reprogrammed human fibroblasts to chemically-induced osteogenic cells
- Dr. Jiyoung-Yoon at ITREN successfully reprogrammed human fibroblasts to chemically-induced osteogenic cells (ciOG). The study recently got published in journal Biomaterials (IF:15.304) under the title “Chemically-induced osteogenic cells for bone tissue engineering and disease modeling".
Reprogrammed cells have been used as platforms for various disease modeling and drug discovery applications because the sources of disease-specific human cells and tissues are often limited. Chemical-induced cell reprogramming has several advantages over the genetic methods such as better safety, expandability, and reproducibility. Inspired from this, Dr. Ji-young Yoon at ITREN successfully reprogrammed human fibroblasts to chemically-induced osteogenic cells (ciOG) by means of a chemical cocktail (RepSox, forskolin, and phenamil) treatment. Various in-vitro studies were performed, especially RNA sequencing analysis were done to reveal distinct ciOG population and its dependence on BMP signaling. In-vivo experiments showed the potential of ciOGs to induce mineralization at ectopic sites. Further engineered nanofibers with ciOGs were utilized to reveal their osteogenic capacity in a critical sized bone defect model. All the results demonstrated that ciOG significantly accelerated neo bone formation and promotes the osteogenic capacity. Additionally, the ciOG platform reviewed the proteus syndrome (PS) and osteogenesis imperfecta (OI) which are genetic bone diseases, allowing candidate drug testing for bone disease modeling and drug discovery.
Read the full article online:
- (2020.10. People) Prof. Hae-Won Kim and Dr. Jung-Hwan Lee reviewed biomaterials roles for promoting angiogenesis in tissue regeneration.
Prof. Hae-Won Kim and Dr. Jung-Hwan Lee reviewed biomaterials roles for promoting angiogenesis in tissue regeneration. A review article entitled “Materials roles for promoting angiogenesis in tissue regeneration” has been published in Progress in Materials Science (IF 30.5).
(see the article: https://www.sciencedirect.com/science/article/pii/S0079642520300967).
In collaboration with Prof. Jonathan C. Knowles at UCL Eastman Dental Institute (UK) and Dr. Prakash Parthibana in ITREN, they reviewed recently highlighted therapeutic roles of biomaterials for accelerating angiogenesis in tissue regeneration.
Enabling angiogenesis is critical for the success of tissue repair therapies and the fate of tissue-engineered constructs.
Although many biochemical signaling molecules have been used, their biological functions in vivo are known to be limited, mainly due to their short lifetime and poor activity.
Matrices (or engineered biomaterials), beyond the biochemical signals, play pivotal roles in stimulating angiogenic processes with long-lasting efficacy with great angiogenic activity.
In this review, they discussed the proangiogenic effort taken to repair and regenerate various tissues including skin, bone, muscle and nerve, focusing on the roles of engineered matrices. This includes the design of pore structure and physico-chemical properties (nanotopology, stiffness, chemistry and degradability), the tailoring of matrices for proper presentation of growth factors and their crosstalks with adhesion ligands, the controlled and sustained delivery of angiogenic molecules and metallic ions, and the engineering of cells and construction of prevascularized tissues.
- (2020.07. People) Dr Hye Sung Kim significantly improved a repair of osteochondral defects using a cartilage-mimicking scaffold
- Dr. Hye Sung Kim significantly improved a repair of osteochondral defects using a cartilage-mimicking scaffold
Dr. Hye Sung Kim at ITREN significantly improved a repair of osteochondral defects using a cartilage-mimicking scaffold. A research article entitled “Protein-reactive nanofibrils decorated with cartilage-derived decellularized extracellular matrix for osteochondral defects” has been published in Biomaterials (IF 10.273)
(see the article: https://www.sciencedirect.com/science/article/pii/S0142961220304609).
In collaboration with Prof. Kam W. Leong at Columbia University (USA) and Prof. Hyuk Sang Yoo at Kangwon National University (South Korea), she has developed a cartilage-mimicking scaffold based on an electrospun nanofiber-based 3-D scaffolds, named “nanofibril”. Cartilage is an avascular tissue and thus it barely regenerates once is damaged. So far stem cell therapy is the best hope but the clinical application is limited due to the poor cell survival in the body. To overcome this issue, her team provided stem cells a cartilage-specific microenvironment in 3-D using nanofibril. The surface of the scaffold was decorated with bovine cartilage-derived decellularized extracellular matrix (dECM) which replicates compositional and structural features of the cartilage tissue. The significance of this study is that the amount of dECM chemically immobilized on the scaffolds is largely improved via a robust surface modification method called surface-initiated atom transfer radical polymerization (SI-ATRP). The cartilage-dECM decorated nanofibril not only improved the cell adhesion and proliferation, but also stimulated the chondrogenic differentiation of adipose-derived mesenchymal stem cells, leading to an improved repair of osteochondral defects.
She said “I’m thrilled to report this exciting results in such an impactful journal in biomedical field. I could not do it without the collaborator’s help. I would really love to continue active collaboration with global institutes here at ITREN”
(See her interview with BRIC: https://www.ibric.org/myboard/read.php?Board=tr_interview&id=211527&qinterview=Y).