In article number 2304821, Rajendra K. Singh, Jung-Hwan Lee, Hae-Won Kim, and co-workers demonstrate that biophysical multiple cues coordinated from electromagnetized Au-nanoparticles-decorated polymer nanofiber under electromagnetic fields significantly regulate the adhesion, alignment, proliferation, and lineage commitment of hMSCs. Without the EMF, matrix cues of electrical conductivity and nanodotted fibrous topography accelerate the anchorage and spreading of hMSCs.