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44 - Synergistic Regulation of Myoblast Cells by Electro-Conductive and Topographic Cues

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Speaker : Hee Seok Yang, Ph.D. (Department of Bioengineering Center for Cardiovascular Biology Institute of Stem Cell and Regenerative Medicine University of Washington) 

Date : 2013-01-22

Location : Room 106 Pharmacy Hall, Dankook University

Abstract : Electrically conductive materials provide a platform for the in vitro study of excitable cells, including skeletal myoblast, due to their inherent conductivity and electro activity. We hypothesize that nanopatterned substrates coated in electrically conductive materials will enhance myoblast differentiation and orientation without the need for electrical stimulation or mechanical stress. In this study, we developed a means to coat polyurethane substrates with electrically conductive materials via an electro-beam evaporator.C2C12 cells, a myoblast cell line, were used to assess cell behavior and skeletal muscle differentiation on unpatterned and patterned electrically conductive substrates. Myotube formation and myoblast differentiation gene expression were also assessed on the unpatterned and patterned electrically conductive substrates. We found that patterned substrates enhanced differentiation of linearly aligned myoblasts, suggesting their suitability as a biomimic substrate for in vitro engineering of skeletal muscle tissue.

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